Q. Which one is a true statement regarding DNA polymerase used in PCR?

Q. Which one is a true statement regarding DNA polymerase used in PCR?

(a) It is used to ligate introduced DNA in recipient cells.

(b) It serves as a selectable marker.

(c) It is isolated from a virus.

(d) It remains active at high temperature.

Solution :

Correct option : D The primary requirements for a DNA polymerase used in PCR are optimal activity at temperatures around 75oC and the ability to retain that activity after prolonged incubation at even higher temperatures (95oC). The first thermostable DNA polymerase to be widely used for PCR is Taq DNA Polymerase.