Q. Which is not necessary for obtaining protoplasts for somatic hybridisation?
(a) Cellulose
(b) Primase
(c) Pectinase
(d) Both (a) and (c)
Solution : Correct option : B Somatic hybridization is the in vitro fusion of isolated protoplasts to form a hybrid cell and its subsequent development to form a hybrid plant. For the enzymatic isolation, a safe method is carried out in a simple solution with a high osmotic pressure along with cell wall degrading enzymes like cellulase and pectinase. Primase is not required.