Q. DNA fragments generated by the restriction endonucleases in a chemical reaction can be separated by :
- Polymerase chain reaction
- Electrophoresis
- Restriction mapping
- Centrifugation
Solution :
Correct option : B A restriction enzyme (or restriction endonuclease) is an enzyme that cuts DNA at or near specific recognition nucleotide sequences known as restriction sites. The DNA fragments are separated by electrophoresis, a process that involves application of an electric field to cause the DNA fragments to migrate into an agarose gel. The gel is then stained with a methylene blue stain to visualize the DNA bands and may be photographed .Thus, the correct answer is option B.
