Q. Commonly used vectors for human genome sequencing are :
(a) T/A cloning vectors
(b) T DNA
(c) BAC and YAC
(d) Expression vectors
Solution :
Correct option : C (a) BAC and YAC stand for bacterial artificial chromosomes and yeast artificial chromosomes respectively. (b)Bacterial artificial chromosomes have the cloning system in the plasmid found inside the Escherichia coli. (c) Long strands of DNA from various sources such as the human genome can be expressed in Escherichia coli. (d)Yeast artificial chromosomes are obtained from the DNA of the yeast, Saccharomyces cerevisiae. (e) Long strands of DNA from various sources can be expressed in yeast too. But some sequences which are unstable or absent when cloned into prokaryotes such as bacterium are found to be stable and intact in YAC clones. Thus, the correct answer is BAC and YAC.
