Q. For its activity nitrogenase requires :

Q. For its activity nitrogenase requires :

1. High input of energy
2. Light
3. Mn²⁺
4. Super oxygen radicals

Solution :

Correct option : A Reduction of inert atmospheric nitrogen to ammonia by living organisms is called biological nitrogen-fixation. Only certain prokaryotic organisms (some bacteria and cyanobacteria) are capable of fixing N₂. Such microbes are called N₂-fixers. These N₂-fixing microbes contain an exclusive enzyme, Nitrogenase that is capable of reducing nitrogen. This enzyme is required to fix atmospheric nitrogen (N≡N). It is made up of Mo-Fe protein. For its activity, nitrogenase requires high input of energy. It catalyzes the conversion of atmospheric nitrogen to ammonia. The synthesis of ammonia requires very high input of energy. It requires 8 ATP for each NH₃ energy. The energy required is obtained from the respiration of the host cells. The overall reaction involved in N₂-fixation is N₂+8e⁻+8H⁺+16ATP→₂NH₃+H₂+16ADP+16P_i